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| Status |
Public on Jun 16, 2010 |
| Title |
Expression data from the PVD and OLL neurons in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Nociceptive neurons develop a complex dendritic arbor to sense noxious stimuli, which enables animals to react to environmental insults and perform self-protective behaviours. The genetic programs controlling neuronal dendritic morphogenesis are poorly understood. In C. elegans, the PVD sensory neuron generates a complex dendritic arbor that envelops the body of the animal. This nociceptive neuron enables study of dendrite formation in vivo.
We used expression arrays to identify genes highly enriched in the PVD neuron while undergoing dendritic morphogenesis. These genes could function in pathways controlling dendrite formation.
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| Overall design |
We employ the mRNA-tagging method to isolate poly(A) RNA from the PVD and OLL neurons by expressing a 3X FLAG-tagged poly(A) binding protein PAB-1 in PVD and OLL under control of the ser-2prom3B promoter. The enriched poly(A) RNA is amplified using the NuGEN WT-Pico amplification system and applied to Affymetrix C. elegans gene expression microarrays. A reference sample was obtained by isolating total RNA from whole animals at the L3/L4 stages and processed as above.
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| Contributor(s) |
Watson JD, Smith CJ, Spencer WC, O'Brien T, Cha B, Albeg A, Treinin M, Miller III DM |
| Citation(s) |
20537990 |
| |
| Submission date |
Apr 01, 2010 |
| Last update date |
Jul 06, 2016 |
| Contact name |
William Clay Spencer |
| E-mail(s) |
[email protected]
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| Phone |
4409356643
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| Organization name |
Vanderbilt University
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| Department |
Cell & Developmental Biology
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| Lab |
David Miller
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| Street address |
465 21st Ave S
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (6)
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| GSM529725 |
PVD and OLL neurons, biological rep1 |
| GSM529726 |
PVD and OLL neurons, biological rep2 |
| GSM529727 |
PVD and OLL neurons, biological rep3 |
| GSM529728 |
L3/L4 stage whole animal reference, rep1 |
| GSM529729 |
L3/L4 stage whole animal reference, rep2 |
| GSM529730 |
L3/L4 stage whole animal reference, rep3 |
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| Relations |
| BioProject |
PRJNA126615 |
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