|
| Status |
Public on May 14, 2010 |
| Title |
MIRNA Gene Evolution in Arabidopsis lyrata and Arabidopsis thaliana: 454 sequencing |
| Organisms |
Arabidopsis lyrata; Capsella rubella |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Deep sequencing of small RNA from three closely related brassicaceae A. thaliana, A. lyrata and Capsella rubella was done to systematically analyze the evolution of MIRNA genes and their targets.
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| Overall design |
Small RNA were extracted from A. lyrata and C. rubella total RNA by size fractionation and converted to DNA amplicons by serial adaptor ligation to both ends followed by RT-PCR. DNA amplicons were sequenced using an Ilumina Genome Analyzer. Resulting sequences were computationally trimmed to remove 3' adaptor sequences.
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| Contributor(s) |
Fahlgren N, Jogdeo S, Kasschau KD, Sullivan CM, Chapman EJ, Laubinger S, Smith LM, Dasenko M, Givan SA, Weigel D, Carrington JC |
| Citation(s) |
20407027 |
| |
| Submission date |
Mar 05, 2010 |
| Last update date |
Jun 11, 2013 |
| Contact name |
James C Carrington |
| E-mail(s) |
[email protected]
|
| Phone |
314-587-1202
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
James C. Carrington
|
| Street address |
975 North Warson Road
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
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| Platforms (2) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE20662 |
MIRNA Gene Evolution in Arabidopsis lyrata and Arabidopsis thaliana |
|
| Relations |
| BioProject |
PRJNA129615 |
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