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| Status |
Public on Apr 08, 2010 |
| Title |
KLF1/EKLF regulatory networks in primary erythroid cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
KLF1 (EKLF) regulates a diverse suite of genes to direct erythroid cell differentiation from bi-potent progenitors. To determine the local cis-regulatory contexts and transcription factor networks in which KLF1 operates, we performed KLF1 ChIP-seq in the mouse. We found at least 945 sites in the genome of E14.5 fetal liver erythroid cells which are occupied by endogenous KLF1. Many of these recovered sites reside in erythroid gene promoters such as β-globin, but the majority are distant to any known gene. Our data suggests KLF1 directly regulates most aspects of terminal erythroid differentiation including production of α and β-globin protein chains, heme biosynthesis, co-ordination of proliferation and anti-apoptotic pathways, and construction of the red cell membrane and cytoskeleton by functioning primarily as a transcriptional activator. Additionally, we suggest new mechanisms for KLF1 co-operation with other transcription factors, in particular the erythroid transcription factor GATA1, to maintain homeostasis in the erythroid compartment.
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| Overall design |
Examination of KLF1 occupancy in primary erythroid cells. KLF1-ChIP and input samples were run on AB SOLiD Systems 2.0 and 3.0. The genomic alignment files (*sorted.txt) and peak file (*bed) contain the combined System 2.0 and 3.0 data.
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| Contributor(s) |
Tallack MR, Whitington T, Yuen WS, Wainwright EN, Keys JR, Gardiner BB, Nourbakhsh E, Cloonan N, Grimmond SM, Bailey TL, Perkins AC |
| Citation(s) |
20508144 |
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| Submission date |
Feb 22, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew C Perkins |
| E-mail(s) |
[email protected]
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| Phone |
+61733462077
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| Fax |
+61733462101
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| Organization name |
The University of Queensland
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| Department |
Institute for Molecular Bioscience
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| Street address |
Institute for Molecular Bioscience
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| City |
The University of Queensland, St Lucia, Brisbane |
| State/province |
QLD |
| ZIP/Postal code |
4072 |
| Country |
Australia |
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| Platforms (2) |
| GPL9318 |
AB SOLiD System 3.0 (Mus musculus) |
| GPL10279 |
AB SOLiD System 2.0 (Mus musculus) |
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| Samples (4)
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| Relations |
| SRA |
SRP002233 |
| BioProject |
PRJNA125163 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20478_EKLF_Peaks_5-fold_withScores_mm9.bed.gz |
8.4 Kb |
(ftp)(http) |
BED |
| GSE20478_Merged_EKLF_mm9_sorted.txt.gz |
56.7 Mb |
(ftp)(http) |
TXT |
| GSE20478_Merged_Input_mm9_sorted.txt.gz |
141.3 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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