|
| Status |
Public on May 12, 2022 |
| Title |
RNA sequencing of 16D LTenza cells transduced with cMyc, shRb1, shTp53, shRb1_shTp53 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to determine how genetic alterations to AR inhibition-maintained prostate cancer cells alters transcriptional programs. We explored how MYC overexpression alters the transcriptional program of 16D cells maintained in enzalutamide for at least 2 months (LTenza) by transducing LTenza 16D cells with control (FUCRW) or MYC. To explore the effect of RB1 and TP53, we generated LTenza 16D cells with knockdown of RB1 and/or TP53 and compared these lines to control-transduced (shScr) LTenza 16D cells. Enzalutamide-naïve (DMSO-treated) cells were included to validate enzalutamide-induced inhibition of AR signaling in enzalutamide-maintained lines.
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| |
|
| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for untransduced DMSO-treated 16D cells and LTenza 16D cells transduced with shScr, FUCRW, MYC, shRB1, shTP53, or shRB1_shTP53
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| |
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| Contributor(s) |
Goldstein AS, Crowell PD |
| Citation(s) |
37815914 |
| |
| Submission date |
May 12, 2022 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Andrew Scott Goldstein |
| Organization name |
UCLA
|
| Department |
Urology
|
| Lab |
Goldstein
|
| Street address |
610 Charles E Young Dr East
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (21)
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|
| Relations |
| BioProject |
PRJNA837604 |
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