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| Status |
Public on Apr 23, 2022 |
| Title |
RESTRICTION checkpoint controls bradyzoite development in Toxoplasma gondii |
| Organism |
Toxoplasma gondii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The Toxoplasma gondii G1 RESTRICTION checkpoint operates the switch between parasite growth and differentiation. The Cdk-related G1 kinase TgCrk2 forms alternative complexes with atypical cyclins (TgCycP1, TgCycP2 and TgCyc5) in the rapidly dividing developmentally incompetent RH and slower dividing developmentally competent ME49 tachyzoites and bradyzoites. The TgCycP1 expression interferes with bradyzoite differentiation. The TgCycP2 regulates G1 in the developmentally competent ME49 but not in the developmentally incompetent RH tachyzoites. Examination of TgCycP2 and TgCyc5 in alkaline induced and spontaneous bradyzoite differentiation (rat embryonic brain cells) models confirmed TgCycP2 role in bradyzoite replication and revealed that stress induced TgCyc5 is critical for efficient tissue cyst maturation.
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| Overall design |
Total RNA was extracted from the purified tachyzoites and bradyzoites of the ME49DKu80Dhxgprt AtTIR1 strain and derivatives. RNA-Seq Library were prepared and sequenced according to manufacturer’s instructions (Illumina).
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| Contributor(s) |
Suvorova ES, Keller T, Kim K, Ting L |
| Citation missing |
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| Submission date |
Apr 18, 2022 |
| Last update date |
Apr 23, 2022 |
| Contact name |
Elena Suvorova |
| E-mail(s) |
[email protected]
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| Organization name |
University of South Florida
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| Street address |
IRB 3720 Spectrum Blvd Suite 304
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| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
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| Platforms (1) |
| GPL25973 |
Illumina NextSeq 500 (Toxoplasma gondii) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA827772 |
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