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| Status |
Public on Mar 23, 2023 |
| Title |
Determination of MHV RNA poly(A) tail length and 3' end additions upon NCTC cells infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The mouse hepatitis virus (MHV) genomic and sub-genomic RNAs have 3’ poly(A) tails. The terminal addition of uridines to poly(A) tails has been shown to be a widespread modification. Here, we investigated the presence of 3' end additions on the MHV RNA poly(A) tails. To this end, we infected NCTC cells with MHV and isolated RNA at 24-hours post-infection (hpi). While the median poly(A) tail length of the MHV RNAs is around 50 nucleotides, we observed a peak of uridylation in transcripts with poly(A) tails about 40 nucleotides long
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| Overall design |
Direct RNAseq of RNA isolated from MHV-infected NCTC cells at 24 hpi
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| Contributor(s) |
Gupta A, Papas BN, Morgan M |
| Citation(s) |
37085578 |
| |
| Submission date |
Apr 07, 2022 |
| Last update date |
May 12, 2023 |
| Contact name |
ANKIT GUPTA |
| E-mail(s) |
[email protected]
|
| Organization name |
NIEHS
|
| Department |
RDBL
|
| Street address |
TW 111 ALEXANDER DRIVE
|
| City |
DURHAM |
| ZIP/Postal code |
27713 |
| Country |
USA |
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| Platforms (1) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE200416 |
Determination of MHV RNA poly(A) tail length and 3' end additions in infected 17-CL1 and NCTC cells |
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| Relations |
| BioProject |
PRJNA824471 |
