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| Status |
Public on Jan 27, 2010 |
| Title |
Expression data from Arabidopsis seed compartments at the bending coyledon stage |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected bending-cotyledon seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing bending cotyledon stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, mycropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, in addition to serial sections encompasing the entire bending-cotyledon stage seed.
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| Overall design |
Bending-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).
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| Contributor(s) |
Kirkbride RC, Belmonte MF, Fei J, Hashimoto MM, Stone SL, Pelletier J, Cheng C, Golberg RB, Harada JJ |
| Citation(s) |
23319655, 25684030 |
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| Submission date |
Jan 26, 2010 |
| Last update date |
Aug 15, 2018 |
| Contact name |
John J Harada |
| E-mail(s) |
[email protected]
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| Phone |
530-752-0673
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| Organization name |
University of California, Davis
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| Department |
Plant Biology
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| Lab |
Harada
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE12404 |
Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development |
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| Relations |
| BioProject |
PRJNA124299 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20039_RAW.tar |
26.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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