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| Status |
Public on Mar 19, 2022 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Drosophila larval Wild Type and TFAM RNAi (r4-gal4>TFAM RNAi) Fat body Transcriptomes |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goal of this RNA-Seq is to analyze the transcriptomes from wild type and TFAM RNAi fat body samples at 96 hr AEL (after egg laying) and to identify the differentially expressed genes.
Methods: Drosophila larval fat bodies were isolated from wild type and TFAM RNAi (r4-gal4) animals at 96 hrs AEL (after egg laying) of development. mRAN profiles were generated by Next Generation Sequencing Facilities.
Results: RNA Seq differential expression analysis identified a significant change in 1758 transcripts, with 1213 transcripts showing reduced expression levels and 545 transcripts with elevated expression levels.
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| Overall design |
Drosophila larval fatbody mRNA profiles of wild type and TFAM RNAi
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| Contributor(s) |
Grewal SS, Sriskanthadevan-Pirahas S |
| Citation(s) |
35545043 |
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| Submission date |
Mar 14, 2022 |
| Last update date |
Jun 20, 2022 |
| Contact name |
Savraj Grewal |
| E-mail(s) |
[email protected]
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| Organization name |
University of Calgary
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| Street address |
3330 Hospital Drive
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| City |
Calgary |
| State/province |
Alberta |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA816098 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198604_gene_lrt_sig.txt.gz |
129.1 Kb |
(ftp)(http) |
TXT |
| GSE198604_tpm_anno_TFAM_RNAi.xlsx |
4.0 Mb |
(ftp)(http) |
XLSX |
| GSE198604_transcript_lrt_wd_sig_anno_fbgn_ipa.xlsx |
179.4 Kb |
(ftp)(http) |
XLSX |
| GSE198604_transcript_wd2.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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