 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2009 |
| Title |
P19 cells+LIN28_RA-differentiated_Day 4 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
LIN28 is an RNA-binding protein expressed in many developing tissues. It can block let-7 microRNA processing and help promote pluripotency. We observe LIN28 expression in the developing neural tube, colocalizing with SOX2, suggesting a role in neural development. To better understand its normal developmental function, we investigated LIN28 activity during neurogliogenesis in vitro where the succession of neuronal to glial cell fates occurs as it does in vivo. LIN28 expression was high in undifferentiated cells, and was down-regulated rapidly upon differentiation. Constitutive LIN28 expression caused a complete block of gliogenesis and an increase in neurogenesis. LIN28 expression was compatible with neuronal differentiation and did not increase proliferation. LIN28 caused significant changes in gene expression prior to any effect on let-7, notably on Igf2. Furthermore, a mutant LIN28 that permitted let-7 accumulation was still able to completely block gliogenesis. Thus, at least two biological activities of LIN28 are genetically separable and may involve distinct mechanisms. LIN28 can differentially promote and inhibit specific fates and does not function exclusively by blocking let-7 family miRNAs. Importantly, LIN28’s role in cell fate succession in vertebrate cells is analogous to its activity as a developmental timing regulator in C. elegans.
|
| |
|
| Overall design |
Two repeats of two cell lines induced to differentiate with retinoic acid/aggregation for four days. Control cells: unmodified mouse P19 embryonal carcinoma cells. Experimental cells: P19 cells constitutively expressing human LIN28A.
|
| |
|
| Contributor(s) |
Balzer E, Heine C, Jiang Q, Lee VM, Moss EG |
| Citation(s) |
20179095 |
| |
| Submission date |
Dec 30, 2009 |
| Last update date |
May 04, 2018 |
| Contact name |
Eric G Moss |
| E-mail(s) |
[email protected]
|
| Organization name |
UMDNJ
|
| Department |
Molecular Biology
|
| Street address |
UMDNJ-SOM/GSBS
|
| City |
Stratford |
| State/province |
NJ |
| ZIP/Postal code |
08084 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (4)
|
| GSM491750 |
P19 cells plus LIN28 - 4 days, rep2 |
|
| Relations |
| BioProject |
PRJNA121889 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19705_RAW.tar |
8.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...