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Status |
Public on Jan 09, 2010 |
Title |
Seed development |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
Reciprocal crosses of a diploid Arabidopsis with different ploidies results in different endosperm development patterns and phenotype. Typically if the ploidy is higher on the maternal side, there are fewer endosperm cells which cellularize early, and conversely, if the paternal ploidy is higher, there is more number of endosperm cells which cellularize late. Crosses involving diploid and tetraploid Arabidopsis (C24) are viable, whereas crosses involving the diploid and hexaploid, even though exhibit the above mentioned directional trend in endosperm development, abort (Scott et al 1998). The 'maternalised' and 'paternalised' development of endosperm is also observed in crosses involving some Arabidopsis mutants. Mutants in the fis class of genes, e.g. fis1-medea, when crossed with a diploid Arabidopsis (pollen parent) show endosperm development-seed development similar to a diploid (seed parent) crossed with hexaploid pollen parent. Reciprocal crosses of homozygous met1 and diploid Arabidopsis also exhibit reciprocal trends in endosperm development, where a homozygous met1 mutant (seed parent) crossed with diploid is similar in phenotype to a diploid (seed parent) - tetraploid cross. Endosperm development in the reciprocal cross has phenotypic similarity to the tetraploid (seed parent)-diploid cross (Adams et al 2000). We are interested in understanding gene profiles and trends in expression underlying the endosperm development in the interploidy crosses as well as the fis and met1 mutant.
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Overall design |
11 samples were used in this experiment.
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Contributor(s) |
Tiwari S |
Citation missing |
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Submission date |
Dec 02, 2009 |
Last update date |
Jun 12, 2017 |
Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
E-mail(s) |
[email protected]
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Phone |
+44 (0)115 951 3237
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Fax |
+44 (0)115 951 3297
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URL |
http://arabidopsis.info/
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Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
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Department |
School of Biosciences, University of Nottingham
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Street address |
Sutton Bonington Campus
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City |
Loughborough |
ZIP/Postal code |
LE12 5RD |
Country |
United Kingdom |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (11)
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GSM477873 |
Tiwari_2-7_2x-X-2x-5dap_Rep1_ATH1 |
GSM477874 |
Tiwari_2-6_fis1-X-2x-2-5dap_Rep2_ATH1 |
GSM477875 |
Tiwari_2-5_fis1-X-2x-1-5dap_Rep1_ATH1 |
GSM477876 |
Tiwari_2-4_2x-X-met1-2-5dap_Rep2_ATH1 |
GSM477877 |
Tiwari_2-3_2x-X-met1-1-5dap_Rep1_ATH1 |
GSM477878 |
Tiwari_2-2_met1-X-2x-2-5dap_Rep2_ATH1 |
GSM477879 |
Tiwari_2-1_met1-X-2x-1-5dap_Rep1_ATH1 |
GSM477880 |
Tiwari_2-8_2x-X-4x-5dap_Rep1_ATH1 |
GSM477881 |
Tiwari_2-9_4x-X-2x-5dap_Rep1_ATH1 |
GSM477882 |
Tiwari_2-10_2x-X-6x-5dap_Rep1_ATH1 |
GSM477883 |
Tiwari_2-11_6x-X-2x-5dap_Rep1_ATH1 |
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Relations |
BioProject |
PRJNA120379 |