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| Status |
Public on Aug 21, 2022 |
| Title |
Gene expression of THP-1 macrophages co-cultured with F. nucleatum under iron-deficient and iron-overload conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Fusobacterium nucleatum (F. nucleatum) contributes to the development of colorectal cancer (CRC) by inducing chronic inflammation. We found that iron deposition in macrophages is associated with poor prognosis of CRC patients with high amount of F. nucleatum. To explore the impact of iron on macrophage properties in the presence of F. nucleatum, we conducted RNA sequencing of THP-1 macrophages pretreated with ferric anmonium citrate (FAC) or deferoxamine (DFO), followed by treatment with F. nucleatum. Several chemokine genes were differentially increased in FAC-treated cells when compared with DFO-treated cells, suggesting that iron is required for the efficient induction of tumor-promoting chemokines in macrophages upon F. nucleatum infection. Our results suggest a prognostic role for iron in F. nucleatum-positive CRC.
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| Overall design |
RNA sequenscing of THP-1 macrophages pretreated with ferric anmonium citrate (FAC) or deferoxamine (DFO), followed by treatment with Fusobacterium nucleatum
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| Contributor(s) |
Yamane T, Kanamori Y, Usuki S, Moroishi T |
| Citation(s) |
36136589 |
| |
| Submission date |
Dec 21, 2021 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Taishi Yamane |
| E-mail(s) |
[email protected]
|
| Organization name |
1. Department of Cell Signaling and Metabolic Medicine, Faculty of Life Sciences, Kumamoto University
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| Street address |
1-1-1 Honjo
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| City |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA791341 |
| SRA |
SRP351961 |
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