|
| Status |
Public on Jan 01, 2024 |
| Title |
A cell-free pipeline for recreating methylation patterns radically enhances DNA transformation in bacteria |
| Organism |
Bifidobacterium breve |
| Experiment type |
Other
|
| Summary |
We report a method for bypassing restriction modification systems to increase DNA transformation in batceria and develop a high-throughput way to determine the optimal methylation pattern for DNA transformation.
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| |
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| Overall design |
Submitted barcoded amplicons from transformed Bifidobacterium breve cells for high-throughput Amplicon EZ sequencing performed by Genewiz to determine the frequency of each barcode.
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| |
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| Contributor(s) |
Vento J, Durmusoglu D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 30, 2021 |
| Last update date |
Apr 09, 2024 |
| Contact name |
Chase Beisel |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Institute for RNA-based Infection Research
|
| Street address |
Josef-Schneider-Str. 2 / Bau D15
|
| City |
Wuerzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL31014 |
Illumina HiSeq 2000 (Bifidobacterium breve) |
|
| Samples (8)
|
| GSM5708772 |
Outgrowth_Antibiotics_Added_Immediately [R1-48] |
| GSM5708773 |
Outgrowth_Overnight_Nonselective_1_10_Dilution [R1-v2] |
| GSM5708774 |
Outgrowth_Overnight_Nonselective_1_100_Dilution [R2-v2] |
| GSM5708775 |
Outgrowth_Overnight_Nonselective_1_1000_Dilution [R3-v2] |
| GSM5708776 |
Outgrowth_Overnight_Nonselective_1_10,000_Dilution [R4-v2] |
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| Relations |
| BioProject |
PRJNA784918 |
| SRA |
SRP348638 |
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