|
| Status |
Public on Nov 18, 2009 |
| Title |
Identification of early gibberellin (GA) responsive genes in Arabidopsis hypocotyls |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The aim of the experiment was to identify early gibberellin (GA) responsive genes in the roots of an Arabidopsis GA deficient mutant.The GA deficient mutant used in this study is a transgenic line overexpressing the PcGA2ox1 gene. This mutant has an identical phenotype to ga1-3, but it does not require exogenous GA treatment for germination.Seeds were germinated on 1xMS + 1% (w/v) sucrose plates containing 0.7% gelrite, and grown under continous light. The plates were orientated vertically.After six days growth the plates were treated with or without 5uM GA4 for 0, 30, 60 and 180 minutes.Approximately 400 hypocotyls were harvested per timepoint using a razorblade and after excision the hypocotyls were frozen in liquid nitrogen giving a total of 7 experimental samples (1: untreated, 2: 30 mins GA4, 3: 30 mins untreated, 4: 60 mins GA4, 5: 60 mins untreated, 6: 180 mins GA4, 7: 180 mins untreated. Three biological replicates were performed giving a total of 21 root samples. Total RNA was isolated from the roots using the QIAGEN RNeasy method.
Keywords: Expression profiling by array
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| Overall design |
21 samples were used in this experiment
|
| |
|
| Contributor(s) |
Gronlund A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 12, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
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| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (21)
|
| GSM469825 |
Gronlund_1-21_BR3+GA-180mins_Rep3_ATH1 |
| GSM469826 |
Gronlund_1-20_BR3-180mins_Rep3_ATH1 |
| GSM469827 |
Gronlund_1-19_BR3+GA-60mins_Rep3_ATH1 |
| GSM469828 |
Gronlund_1-18_BR3-60mins_Rep3_ATH1 |
| GSM469829 |
Gronlund_1-17_BR3+GA-30mins_Rep3_ATH1 |
| GSM469830 |
Gronlund_1-16_BR3-30mins_Rep3_ATH1 |
| GSM469831 |
Gronlund_1-15_BR3-0mins_Rep3_ATH1 |
| GSM469832 |
Gronlund_1-14_BR2+GA-180mins_Rep2_ATH1 |
| GSM469833 |
Gronlund_1-13_BR2-180mins_Rep2_ATH1 |
| GSM469834 |
Gronlund_1-12_BR2+GA-60mins_Rep2_ATH1 |
| GSM469835 |
Gronlund_1-11_BR2-60mins_Rep2_ATH1 |
| GSM469836 |
Gronlund_1-10_BR2+GA-30mins_Rep2_ATH1 |
| GSM469837 |
Gronlund_1-9_BR2-30mins_Rep2_ATH1 |
| GSM469838 |
Gronlund_1-8_BR2-0mins_Rep2_ATH1 |
| GSM469839 |
Gronlund_1-7_BR1+GA-180mins_Rep1_ATH1 |
| GSM469840 |
Gronlund_1-6_BR1-180mins_Rep1_ATH1 |
| GSM469841 |
Gronlund_1-5_BR1+GA-60mins_Rep1_ATH1 |
| GSM469842 |
Gronlund_1-4_BR1-60mins_Rep1_ATH1 |
| GSM469843 |
Gronlund_1-3_BR1+GA-30mins_Rep1_ATH1 |
| GSM469844 |
Gronlund_1-2_BR1-30mins_Rep1_ATH1 |
| GSM469845 |
Gronlund_1-1_BR1-0mins_Rep1_ATH1 |
|
| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA120649 |
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