|
| Status |
Public on Nov 18, 2009 |
| Title |
Treatment of Arabidopsis with low concentration of nitrate |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The genomic response to low levels of nitrate was studied in Arabidopsis using the Affymetrix ATH1 chip containing more than 22,500 probe sets. Arabidopsis plants were grown hydroponically in sterile liquid culture on ammonium as the sole source of nitrogen for 10 d, then treated with 250 um nitrate for 20 min. The response to nitrate was much stronger in roots (1,176 genes showing increased or decreased mRNA levels) than in shoots (183 responding genes). In addition to known nitrate-responsive genes (e.g. those encoding nitrate transporters, nitrate reductase, nitrite reductase, ferredoxin reductase, and enzymes in the pentose phosphate pathway), genes encoding novel metabolic and potential regulatory proteins were found. These genes encode enzymes in glycolysis (glucose-6-phosphate isomerase and phosphoglycerate mutase), in trehalose-6-P metabolism (trehalose-6-P synthase and trehalose-6-P phosphatase), in iron transport/metabolism (nicotianamine synthase), and in sulfate uptake/reduction. In many cases, only a few select genes out of several in small gene families were induced by nitrate. These results show that the effect of nitrate on gene expression is substantial (affecting almost 10% of the genes with detectable mRNA levels) yet selective and affects many genes involved in carbon and nutrient metabolism.
Keywords: Expression profilling by array
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| |
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| Overall design |
8 samples were used in this experiment
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| |
|
| Contributor(s) |
Wang R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 12, 2009 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
| Samples (8)
|
| GSM469817 |
Wang_1-1_WT-Col-250-micromolar-KCl-20-min-roots_Rep1_ATH1 |
| GSM469818 |
Wang_1-2_WT-Col-250-micromolar-K-nitrate-20-min-roots_Rep1_ATH1 |
| GSM469819 |
Wang_1-3_WT-Col-250-micromolar-KCl-20-min-roots_Rep2_ATH1 |
| GSM469820 |
Wang_1-4_WT-Col-250-micromolar-K-nitrate-20-min-roots_Rep2_ATH1 |
| GSM469821 |
Wang_1-5_Wild-type-Col-250-micromolar-KCL-20-min-shoots_Rep1_ATH1 |
| GSM469822 |
Wang_1-6_Wild-type-Col-250-micromolar-KNO3-20-min-shoots_Rep1_ATH1 |
| GSM469823 |
Wang_1-7_Wild-type-Col-250-micromolar-KCL-20-min-shoots_Rep2_ATH1 |
| GSM469824 |
Wang_1-8_Wild-type-Col-250-micromolar-KNO3-20-min-shoots_Rep2_ATH1 |
|
| Relations |
| BioProject |
PRJNA120647 |
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