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Status |
Public on Nov 18, 2009 |
Title |
Molecular responses to external pH changes in roots of Arabidopsis thaliana |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
Plants live in soils that vary considerably, both spatially and over time, in terms of nutrient composition and pH. Consistently, plants have to recognize and adapt to these changes by altering their structure and metabolism. The goal of this array analysis is to characterize the global transcriptional response to external pH changes in roots, which to date is almost unexplored. Arabidopsis thaliana (Columbia-0) were grown in hydroponic cultures in basic nutrient solution. Two days before treatment the media was shifted to nutrient solution containing 5mM MES, pH 6. At the time of the treatment start (4 hours after light on) the plants were shifted to nutrient solutions of pH 4.5 and 6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Keywords: Expression profilling by array
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Overall design |
12 samples were used in this experiment
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Contributor(s) |
Lager I |
Citation missing |
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Submission date |
Nov 12, 2009 |
Last update date |
Aug 15, 2018 |
Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
E-mail(s) |
[email protected]
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Phone |
+44 (0)115 951 3237
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Fax |
+44 (0)115 951 3297
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URL |
http://arabidopsis.info/
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Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
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Department |
School of Biosciences, University of Nottingham
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Street address |
Sutton Bonington Campus
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City |
Loughborough |
ZIP/Postal code |
LE12 5RD |
Country |
United Kingdom |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (12)
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Relations |
Affiliated with |
GSE69995 |
BioProject |
PRJNA120973 |