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| Status |
Public on Nov 24, 2009 |
| Title |
Expression data from overexpressers and mutants of TFs-gene LBD37 and LBD38 under different nitrogen regimes |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Nitrogen (N) and nitrate (NO3-) per se regulate many aspects of plant metabolism, growth and development. N/NO3- also suppresses parts of secondary metabolism including anthocyanin synthesis. Molecular components for this repression are unknown. We report that three N/NO3--induced members of the LATERAL ORGAN BOUNDARY DOMAIN (LBD) gene family of transcription factors (LBD37, LBD38 and LBD39) act as negative regulators of anthocyanin biosynthesis in Arabidopsis (Arabidopsis thaliana). Over-expression of each of the three genes in the absence of N/NO3- strongly suppresses the key regulators of anthocyanin synthesis PAP1 and PAP2, genes in the anthocyanin-specific part of flavonoid synthesis, as well as cyanidin- but not quercetin- or kaempferol-glycoside production. Conversely, lbd37, lbd38 or lbd39 T-DNA insertion mutants accumulate anthocyanins when grown in N/NO3--sufficient conditions and show constitutive expression of anthocyanin biosynthetic genes. The LBD genes also repress many other known N-responsive genes including key genes required for NO3- uptake and assimilation, resulting in altered NO3- content, nitrate reductase activity/activation, protein, amino acid and starch levels, and N-related growth phenotypes. The results identify LBD37 and its two close homologs as novel repressers of anthocyanin biosynthesis and N-availability signals in general. They also show that besides being developmental regulators LBD genes fulfill roles in metabolic regulation.
We used microarrays to test whether LBD37 and LBD38 have a more widespread role in NO3-/N-regulation.
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| Overall design |
9-day old Arabidopsis seedlings were harvested for RNA extraction and hybridization on Affymetrix microarrays. Total RNA was prepared separately from three biological replicates of N-limited LBD37OX, LBD38OX and WT seedlings, and N-replete lbd37-1 mutant, lbd38 mutant and WT seedlings (18 samples).
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| Contributor(s) |
Rubin G, Scheible W |
| Citation(s) |
19933203 |
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| Submission date |
Oct 30, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Grit Rubin |
| E-mail(s) |
[email protected]
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| Organization name |
MPI-MP
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| Department |
Stitt
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| Lab |
Scheible
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| Street address |
Am Mühlenberg 1
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| City |
Potsdam |
| ZIP/Postal code |
14467 |
| Country |
Germany |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (18)
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| GSM466621 |
WT seedlings in noN, biological rep1 |
| GSM466622 |
WT seedlings in noN, biological rep2 |
| GSM466623 |
WT seedlings in noN, biological rep3 |
| GSM466624 |
overexpresser of LBD37 seedlings in noN, biological rep1 |
| GSM466625 |
overexpresser of LBD37 seedlings in noN, biological rep2 |
| GSM466626 |
overexpresser of LBD37 seedlings in noN, biological rep3 |
| GSM466627 |
overexpresser of LBD38 seedlings in noN, biological rep1 |
| GSM466628 |
overexpresser of LBD38 seedlings in noN, biological rep2 |
| GSM466629 |
overexpresser of LBD38 seedlings in noN, biological rep3 |
| GSM466630 |
WT seedlings in FN, biological rep1 |
| GSM466631 |
WT seedlings in FN, biological rep2 |
| GSM466632 |
WT seedlings in FN, biological rep3 |
| GSM466633 |
T-DNA-insertion mutant of LBD37 seedling in FN, biological rep1 |
| GSM466634 |
T-DNA-insertion mutant of LBD37 seedling in FN, biological rep2 |
| GSM466635 |
T-DNA-insertion mutant of LBD37 seedling in FN, biological rep3 |
| GSM466636 |
T-DNA-insertion mutant of LBD38 seedling in FN, biological rep1 |
| GSM466637 |
T-DNA-insertion mutant of LBD38 seedling in FN, biological rep2 |
| GSM466638 |
T-DNA-insertion mutant of LBD38 seedling in FN, biological rep3 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA121681 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18818_RAW.tar |
61.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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