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Series GSE18200 Query DataSets for GSE18200
Status Public on Dec 31, 2009
Title Gene expression C. elegans age-1 mutants with neuron- or intestine-restricted age-1 activity
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary The goal of this experiment was to identify transcripts regulated non-autonomously by age-1 activity in neurons or intestine. Comparisons of gene expression were made between age-1(mg44) null adults and CY251 (age-1(mg44); bvIs2) and CY262 (age-1(mg44); bvIs1); all were relative to wildtype (N2). The integrated transgenes, bvIs2 and bvIs1, carry the age-1 cDNA expressed from the neuronal ric-19 promoter (bvIs2) or intestinal ges-1 promoter (bvIs1).
Transcripts upregulated in age-1(mg44) adults compared to wildtype (>=1.5 fold; p<0.05) or downregulated (<0.6 fold; p<0.05) were considered to be age-1 target genes. Rescue in CY251 or CY262 was determined by change in the transgenic background versus age-1(mg44) (all relative to N2 wildtype). Non-autonomous rescue of a target gene was usually determined by transcript levels that were at least 50% rescued compared to wildtype.
 
Overall design Biological replicates: 2 age-1(mg44), 3 CY251 and 3 CY262
 
Contributor(s) Iser WB, Wilson MA, Rao SK, Becker K, Wolkow CA
Citation(s) 21408062
Submission date Sep 21, 2009
Last update date Mar 09, 2020
Contact name Catherine Wolkow
E-mail(s) [email protected]
Organization name NIA IRP, NIH
Street address 251 Bayview Blvd
City Baltimore
State/province MD
ZIP/Postal code 21224
Country USA
 
Platforms (1)
GPL2875 Agilent-012795 C. elegans Oligo Microarray G2518A Option 002 (Feature Number version)
Samples (8)
GSM455141 age-1(mg44)_rep1
GSM455142 intestine_age-1_rep1
GSM455143 neuron_age-1_rep1
Relations
BioProject PRJNA119557

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18200_RAW.tar 40.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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