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Status |
Public on Dec 31, 2009 |
Title |
Gene expression C. elegans age-1 mutants with neuron- or intestine-restricted age-1 activity |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
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Summary |
The goal of this experiment was to identify transcripts regulated non-autonomously by age-1 activity in neurons or intestine. Comparisons of gene expression were made between age-1(mg44) null adults and CY251 (age-1(mg44); bvIs2) and CY262 (age-1(mg44); bvIs1); all were relative to wildtype (N2). The integrated transgenes, bvIs2 and bvIs1, carry the age-1 cDNA expressed from the neuronal ric-19 promoter (bvIs2) or intestinal ges-1 promoter (bvIs1). Transcripts upregulated in age-1(mg44) adults compared to wildtype (>=1.5 fold; p<0.05) or downregulated (<0.6 fold; p<0.05) were considered to be age-1 target genes. Rescue in CY251 or CY262 was determined by change in the transgenic background versus age-1(mg44) (all relative to N2 wildtype). Non-autonomous rescue of a target gene was usually determined by transcript levels that were at least 50% rescued compared to wildtype.
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Overall design |
Biological replicates: 2 age-1(mg44), 3 CY251 and 3 CY262
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Contributor(s) |
Iser WB, Wilson MA, Rao SK, Becker K, Wolkow CA |
Citation(s) |
21408062 |
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Submission date |
Sep 21, 2009 |
Last update date |
Mar 09, 2020 |
Contact name |
Catherine Wolkow |
E-mail(s) |
[email protected]
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Organization name |
NIA IRP, NIH
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Street address |
251 Bayview Blvd
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City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21224 |
Country |
USA |
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Platforms (1) |
GPL2875 |
Agilent-012795 C. elegans Oligo Microarray G2518A Option 002 (Feature Number version) |
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Samples (8)
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Relations |
BioProject |
PRJNA119557 |