|
| Status |
Public on May 12, 2022 |
| Title |
Single-cell RNA-seq of human kidney organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used scRNA-Seq (and other biological experiments) to study the impact of a high oscillatory glucose regime in the differentiation and metabolic status of human kidney organoids further emulating early hallmarks of the diabetic kidney. To study the interplay between those changes and SARS-CoV-2 infection we assessed the effect of early stages of infection (1 day post infection) in normoglicemic (5 mM), hyperglicemic (5-25 mM oscillatory regime) and differentiating (11 mM) conditions. We retrieved metabolic changes and an upregulation of inflammatory related processes upon infection, whilst there was not an impact in the viability of the different cell types in the kidney organoids. Also, we studied the differences between ACE2 CRISPR/Cas9 knockouts and ACE2 WT organoids, using single cell RNA sequencing to resolve cell-type specific and cell proportion changes.
|
| |
|
| Overall design |
Single-cell RNA-seq of human kidney organoids
|
| |
|
| Contributor(s) |
Montserrat N, Ullate-Agote A |
| Citation(s) |
35561674 |
| |
| Submission date |
Jul 28, 2021 |
| Last update date |
May 16, 2022 |
| Contact name |
Asier Ullate-Agote |
| E-mail(s) |
[email protected]
|
| Organization name |
CIMA
|
| Department |
Hemato-Oncology
|
| Lab |
Advanced Genomics
|
| Street address |
Av. de Pío XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (8)
|
| GSM5481909 |
Kidney organoids 5mM glucose mock |
| GSM5481910 |
Kidney organoids 5mM glucose 1dpi SARS-CoV-2 |
| GSM5481911 |
Kidney organoids 5-25mM oscillatory glucose mock |
| GSM5481912 |
Kidney organoids 5-25mM oscillatory glucose 1dpi SARS-CoV-2 |
| GSM5481913 |
Kidney organoids 11mM glucose mock |
| GSM5481914 |
Kidney organoids 11mM glucose 1dpi SARS-CoV-2 |
| GSM5481915 |
Kidney organoids ACE2 WT B10 |
| GSM5481916 |
Kidney Organoids ACE2 KO G7 |
|
| Relations |
| BioProject |
PRJNA750396 |
| SRA |
SRP330212 |
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