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| Status |
Public on Aug 23, 2021 |
| Title |
Distinct Functional Properties of Perinatal vs Adult Adipose Progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously identified functional distinct sub-popultions in adult male gWAT Pdgfrb+ cells utilizing the MuralChaser lineage tracking system and Single-cell RNA-seq. To examine the cellular altas and developmental aspects of male gWAT, we performed Single-cell RNA-seqs on different developing stage of gWAT (P3,P7 and 5-week). To examine the mesothelial origin hypothesis of gWAT, we also performed Single-cell RNA-seq on P7 gWAT associated mesothelial cells.
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| Overall design |
We isolated Stromal Vascular Fraction (SVF) from epidydimal white adipose tissue (eWAT) of Postnatal Day 3 (n=10)/ Psotnatal Day 7 (n=5) / 5-week (n=5) male mice respectively. We also isolated Postnatal Day 7 eWAT associated mesothelial cells. Single cell library preparation was performed using the 10X Genomics Single Cell 3 v3 according to the manufacturers instructions.
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| Contributor(s) |
Gupta R, Zhang Q, Guo L |
| Citation(s) |
35982290 |
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| Submission date |
Jul 28, 2021 |
| Last update date |
Sep 08, 2022 |
| Contact name |
Rana K Gupta |
| E-mail(s) |
[email protected]
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| Organization name |
Duke University
|
| Department |
Duke Molecular Physiology Institute
|
| Street address |
Carmichael Building, 300 North Duke St.
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| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27701 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA750313 |
| SRA |
SRP330183 |
