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| Status |
Public on Sep 12, 2009 |
| Title |
Chloroplast polynucleotide phosphorylase null mutant (pnp1-1) and phosphate starvation |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
A prominent enzyme in organellar RNA metabolism is the exoribonuclease polynucleotide phosphorylase (PNPase), whose reversible activity is governed by the nucleotides diphosphate-inorganic phosphate ratio. In Chlamydomonas reinhardtii, PNPase regulates chloroplast transcript accumulation in response to phosphorus (P) starvation, and PNPase expression is repressed by the response regulator PSR1 under these conditions. Here, we investigated the role of PNPase in the Arabidopsis (Arabidopsis thaliana) P deprivation response by comparing wild-type and pnp mutant plants with respect to their morphology, metabolite profiles, and transcriptomes. We found that P-deprived pnp mutants develop aborted clusters of lateral roots, which are characterized by decreased auxin responsiveness and cell division, and exhibit cell death at the root tips. Electron microscopy revealed that the collapse of root organelles is enhanced in the pnp mutant under P deprivation and occurred with low frequency under P-replete conditions. Global analyses of metabolites and transcripts were carried out to understand the molecular bases of these altered P deprivation responses. We found that the pnp mutant expresses some elements of the deprivation response even when grown on a full nutrient medium, including altered transcript accumulation, although its total and inorganic P contents are not reduced. The pnp mutation also confers P status-independent responses, including but not limited to stress responses. Taken together, our data support the hypothesis that the activity of the chloroplast PNPase is involved in plant acclimation to P availability and that it may help maintain an appropriate balance of P metabolites even under normal growth conditions.
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| Overall design |
For each timepoints (3h and one week), we compared WT and pnp1-1 on +P and on -P, so a total of eight conditions For each condition, three biological replicates of at least 4 seedlings (without roots) were analyzed.
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| Contributor(s) |
Marchive C, Fei Z, Stern DB |
| Citation(s) |
19710229 |
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| Submission date |
Sep 11, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Chloe Marchive |
| E-mail(s) |
[email protected]
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| Phone |
1 607 254 1304
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| Fax |
1 607 255 6695
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| Organization name |
Boyce Thompson Institute
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| Lab |
Stern Lab
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| Street address |
Tower road
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14850 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (24)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA119339 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18071_RAW.tar |
52.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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