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| Status |
Public on Jun 08, 2021 |
| Title |
Modeling pancreatic beta cell senescence by induction of DNA double-strand breaks |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pancreatic beta cell senescence occurs during the development of Type 1 Diabetes. To model the transcriptional responses of islet cells to DNA damage, we previously developed a human islet culture model in which the DNA damage response and senescence can be induced via double strand-breaks with the agent bleomycin. Here, we report the transcriptome-wide changes in human pancreatic islet cells following bleomycin exposure.
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| Overall design |
Human donor islets isolated from a 44 year old normoglycemic female by Scharp-Lacy and distributed by the Integrated Islet Distribution Program (RRID:SAMN13186657) and from a 50 year old normoglycemic female by Alberta Diabetes Institute (RRID:SAMN26419385) were rested 24 h and then treated with vehicle (DMSO) or 50 uM bleomycin for 48 h. Islets from each donor were treated with Veh or Bleo in n=3 biological replicates (wells of islets). Following this, the islets were transferred to fresh drug free media and cultured an additional 4 days before harvest and RNA extraction. Approximately 1 ug of total RNA was submitted to LC Sciences for total RNA-seq on Illumina NovaSeq 6000 platform.
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| Web link |
https://www.sciencedirect.com/science/article/pii/S221287782200093X?via%3Dihub
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| |
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| Contributor(s) |
Thompson PJ, Brawerman G |
| Citation(s) |
35660116 |
| |
| Submission date |
Jun 07, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Peter Thompson |
| E-mail(s) |
[email protected]
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| Organization name |
University of Manitoba
|
| Department |
Physiology & Pathophysiology
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| Street address |
715 McDermot ave
|
| City |
Winnipeg |
| State/province |
MB |
| ZIP/Postal code |
R3E 3P4 |
| Country |
Canada |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA735767 |
| SRA |
SRP323077 |
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