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| Status |
Public on Jan 01, 2023 |
| Title |
Fate restricted stromal fibroblasts and adipocytes demonstrate multi-modal responses to tissue injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Adult connective tissues rearrange themselves during injury, disease, and aging. This response is widely considered to involve cross-lineage conversion between two differentiated stromal cell types: fibroblasts and adipocytes. Here, we directly explore the potential plasticity of these cells after injury in the skin. Using single-cell transcriptomics we confirm that adipocyte and fibroblast lineages undergo separate differentiation pathways after injury, avoiding cross-lineage conversion. We performed Single-Cell RNA-seq experiments and employed a fibroblast-lineage specific reporter (En1Cre), labeling a sub-population of fibroblasts termed EPFs (Engrailed-lineage positive fibroblasts). Mature adipocytes were tracked using an adiponectin lineage-specific reporter (AdipoqCre). The two lineage-specific transgenic lines were individually crossed to a double-color fluorescent reporter (R26mTmG), thereby marking mature adipocytes and fibrogenic cells with membrane-bound green. Sorted populations were subjected to SCRB-seq analysis.
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| Overall design |
Single-cell transcriptomics on mouse stromal cells after injury in the skin
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| Contributor(s) |
Kalgudde Gopal S, Stefanska AM, Ansari M, Schiller HB, Rinkevich Y |
| Citation missing |
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| Submission date |
May 27, 2021 |
| Last update date |
Jan 03, 2023 |
| Contact name |
Meshal Ansari |
| Organization name |
Boehringer Ingelheim
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| Street address |
Birkendorfer Straße 65
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| City |
Biberach |
| ZIP/Postal code |
88397 |
| Country |
Germany |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA733149 |
| SRA |
SRP321616 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE175650_RAW.tar |
94.2 Mb |
(http)(custom) |
TAR (of H5AD, MTX, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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