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| Status |
Public on May 05, 2021 |
| Title |
Transcriptional organization and regulation of the Pseudomonas putida flagellar system |
| Organism |
Pseudomonas putida KT2440 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The entire set of flagellar structural components and flagellar-specific transcriptional regulators, as well as much of the core chemotaxis machinery, is encoded into a >70 kbp cluster in Pseudomonas putida KT2440 genome. We have performed RNA-seq of the wild-type strain in order to identify operon boundaries and promoters location in this cluster.
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| Overall design |
3 biological replicates of wild-type P. putida KT2440 were grown to stationary phase (OD600=2) in Luria-Bertani (LB) broth at 30ºC and 180 rpm shaking.
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| Contributor(s) |
Govantes F, Pulido-Sánchez M, López-Sánchez A |
| Citation(s) |
34859548 |
| |
| Submission date |
May 04, 2021 |
| Last update date |
Jan 12, 2022 |
| Contact name |
Fernando Govantes |
| E-mail(s) |
[email protected]
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| Organization name |
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide
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| Department |
Biología Molecular e Ingeniería Bioquímica
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| Street address |
Carretera de Utrera, Km. 1
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| City |
Sevilla |
| State/province |
Sevilla |
| ZIP/Postal code |
41013 |
| Country |
Spain |
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| Platforms (1) |
| GPL28478 |
Illumina NextSeq 500 (Pseudomonas putida KT2440) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA727301 |
| SRA |
SRP318602 |
