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| Status |
Public on Jul 26, 2021 |
| Title |
Transcriptome analysis of CCR9+, CXCR5+ and CCR9-CXCR5- Th cells in primary Sjögren's syndrome (pSS) patients and healthy controls (HC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CCR9+ Tfh-like pathogenic T helper (Th) cells are elevated in patients with primary Sjögren’s syndrome (pSS) and indicated to play a role in pSS immunopathology. CCR9+, CXCR5+ and CCR9-CXCR5- (double negative, DN) Th cells from blood of 7 healthy controls (HC) and 7 pSS patients were FACS sorted and RNA sequencing was performed. Our aim is to delineate the CCR9+ Th cell-specific transcriptome to study the molecular dysregulation of these cells in pSS patients. Transcriptomic analysis of circulating CCR9+ Th cells reveals CCR9-specific pathways involved in effector T cell function, equally expressed in pSS patients and HC. Given the increased numbers of CCR9+ Th cells in the blood and inflamed glands of pSS patients and presence of inflammatory stimuli to activate these cells this suggests that CCR9-specific functions, such as cell recruitment upon CCL5 secretion, could significantly contribute to immunopathology in pSS.
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| Overall design |
FACS sorted CCR9+, CXCR5+ and CCR9-CXCR5- Th cells from peripheral blood of n=7 pSS patients and n=7 HC were analyzed using RNA sequencing
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| Contributor(s) |
Hinrichs AC, Pandit A, vanRoon JA |
| Citation(s) |
34386009 |
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| Submission date |
Apr 30, 2021 |
| Last update date |
Aug 18, 2021 |
| Contact name |
Anneline Hinrichs |
| Organization name |
University Medical Center Utrecht
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| Department |
Rheumatology & Clinical Immunology
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| Street address |
Postbus 85500
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| City |
Utrecht |
| ZIP/Postal code |
3508 GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA726438 |
| SRA |
SRP318000 |
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