|
| Status |
Public on Jan 01, 2022 |
| Title |
Intergenerational metabolic priming by sperm piRNAs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Preconception parental environment can reproducibly program offspring phenotype without altering the DNA sequence, yet the mechanisms underpinning this ‘epigenetic inheritance’ remains elusive. Here, we demonstrate the existence of an intact piRNA-pathway in mature Drosophila sperm and show that pathway modulation alters offspring gene transcription in a sequence-specific manner. We map a dynamic small RNA content in developing sperm and find that the mature sperm carry a highly distinct small RNA cargo. By biochemical pulldown, we identify a small RNA subset bound directly to piwi protein. And, we show that piRNA-pathway controlled sperm small RNAs are linked to target gene repression in offspring. Critically, we find that full piRNA-pathway dosage is necessary for the intergenerational metabolic and transcriptional reprogramming events triggered by high paternal dietary sugar. These data provide a direct link between regulation of endogenous mature sperm small RNAs and transcriptional programming of complementary sequences in offspring. Thus, we identify a novel mediator of paternal intergenerational epigenetic inheritance.
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| Overall design |
Drosophila melanogaster small RNA and mRNA samples with differing library preparations, tissue, and genotypes.
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| Contributor(s) |
Beddows I, Lempradl H |
| Citation missing |
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| |
| Submission date |
Apr 14, 2021 |
| Last update date |
Jan 01, 2022 |
| Contact name |
Ian Beddows |
| E-mail(s) |
[email protected]
|
| Organization name |
Van Andel Institute
|
| Street address |
333 Bostwick Ave NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA722018 |
| SRA |
SRP314867 |
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