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| Status |
Public on Jul 28, 2021 |
| Title |
Dissecting mechanisms by which MyoD and small molecules convert fibroblasts to muscle progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The generation of myotubes from fibroblasts upon forced MyoD expression is a classic example of factor-induced reprogramming in mammals. We recently discovered that additional modulation of signaling pathways with small molecules facilitates reprogramming to more primitive induced muscle progenitor cells (iMPCs). However, the mechanisms by which a single transcription factor drives differentiated cells into distinct developmental states remain unknown. We therefore dissected the transcriptional and epigenetic dynamics of fibroblasts undergoing MyoD-dependent reprogramming to either myotubes or iMPCs using a novel MyoD transgenic model. To this end, we performed single cell RNA sequencing for Pax7-nGFP positive iMPCs/satellite cells and cells undergoing dedifferentiation (i.e. Dox+FRG) or transdifferentiation (i.e. Dox) Our analyses elucidate the role of MyoD in myogenic reprogramming and derive general principles by which transcription factors and signaling pathways cooperate to rewire cell identity. Our results may also inform on potential therapeutic applications of direct reprogramming.
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| Overall design |
single cell RNA-seq was performed for 10 samples
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| |
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| Contributor(s) |
Yagi M, Cristea S, Michor F, Hochedlinger K |
| Citation(s) |
34413137 |
| |
| Submission date |
Mar 29, 2021 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Simona Cristea |
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Biostatistics and Computational Biology
|
| Street address |
450 Brookline Ave.
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA718201 |
| SRA |
SRP312561 |
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