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| Status |
Public on Apr 27, 2021 |
| Title |
Identification of therapeutic targets of the hijacked super-enhancer complex in EVI1-rearranged leukemia (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Chromosomal aberrations in acute myeloid leukemia (AML), such as inv(3) and t(3;3), lead to deregulation of the EVI1 oncogene by the GATA2 distal hematopoietic enhancer (G2DHE). In this project, we aimed to study the transcription factor complexes involved in the regulation of the G2DHE sequence. We have identified PARPi as a member of the G2DHE complex. Here, we used RNA-Seq to analyze transcriptomic changes after PARP inhibition with olaparib and talazoparib and to compare those to EVI1 knockdown.
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| |
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| Overall design |
RNA-Seq in MUTZ-3 cells with PARP inhibition or EVI1 knockdown.
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| |
|
| Contributor(s) |
Herrmann C, Kiehlmeier S, Gröschel S |
| Citation(s) |
33911178 |
| |
| Submission date |
Feb 17, 2021 |
| Last update date |
May 01, 2021 |
| Contact name |
Stefan Gröschel |
| Organization name |
DKFZ German Cancer Research Center
|
| Street address |
Im Neuenheimer Feld 581
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE153307 |
Identification of therapeutic targets of the hijacked super-enhancer complex in EVI1-rearranged leukemia |
|
| Relations |
| BioProject |
PRJNA702595 |
| SRA |
SRP306923 |
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