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| Status |
Public on Feb 17, 2021 |
| Title |
Gene expression after knockdown of transcription factors in human iPSC-derived cardiac myocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report gene expression changes after knockdown of transcription factors in human iPSC-derived cardiac myocytes. In prior experiments we showed that transcription factors known to be important for cardiac development were frequently up-regulated (i.e., "responsive") after small molecule perturbations of cultured iPSC-derived cardiac myocytes. We used RNA sequencing to test whether siRNA-mediated knockdown of transcription factors with different perturbation-responsiveness and tissue-specificity profiles would lead to inappropriate up-regulation of non-myocyte gene sets in cardiac myocytes.
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| Overall design |
Paired-end RNA sequencing of 9 different targeting siRNA conditions, 1 scrambled siRNA control condition, and 1 non-transfected control condition. All conditions in triplicate. 33 total samples.
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| Contributor(s) |
Mellis IA, Jain R, Raj A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 16, 2021 |
| Last update date |
Feb 17, 2021 |
| Contact name |
Ian A Mellis |
| E-mail(s) |
[email protected]
|
| Organization name |
Columbia University
|
| Street address |
622 W 168th St
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
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| Samples (33)
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| Relations |
| BioProject |
PRJNA702074 |
| SRA |
SRP306591 |
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