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| Status |
Public on Feb 13, 2021 |
| Title |
Lactobacillus Firm5 in vivo and in vitro RNA sequencing |
| Organisms |
Lactobacillus apis; Lactobacillus kullabergensis; Lactobacillus helsingborgensis; Lactobacillus melliventris |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the transcriptome of four strains of the Lactobacillus Firm5 phylotype obtained with in vivo and in vitro experiments under different nutrient conditions
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| Overall design |
In vivo experiment: microbiota depleted bees were colonized with the four strains community and divided into a treatment without access to pollen and a treatment with access to pollen. In vitro experiment: Firm5 strains were cultures as a community as well as mono-cultures in presence or absence of pollen. We compare gene expression between treatments to understand the impact of diet and presence of other strains on gene expression.
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| Contributor(s) |
Brochet S, Engel P |
| Citation missing |
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| |
| Submission date |
Feb 12, 2021 |
| Last update date |
Feb 15, 2021 |
| Contact name |
Silvia Brochet |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Lausanne
|
| Department |
Department of Fundamental Microbiology
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| Lab |
Engel
|
| Street address |
UNIL campus, Biophore building
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| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
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| Platforms (6)
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| GPL29719 |
Illumina MiniSeq (Lactobacillus helsingborgensis) |
| GPL29720 |
Illumina MiniSeq (Lactobacillus melliventris) |
| GPL29721 |
Illumina MiniSeq (Lactobacillus apis) |
| GPL29722 |
Illumina MiniSeq (Lactobacillus kullabergensis) |
| GPL29723 |
Illumina HiSeq 4000 (Lactobacillus apis; Lactobacillus helsingborgensis; Lactobacillus kullabergensis; Lactobacillus melliventris) |
| GPL29724 |
Illumina MiniSeq (Lactobacillus apis; Lactobacillus helsingborgensis; Lactobacillus kullabergensis; Lactobacillus melliventris) |
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| Samples (49)
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| Relations |
| BioProject |
PRJNA701689 |
| SRA |
SRP306264 |
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