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| Status |
Public on Aug 27, 2021 |
| Title |
Profiling of the chromatin landscape of hematopoietic progenitor cells (HPC) transduced with transcription factors T-BET and EOMES [ATAC-seq HPC] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
T-BET and EOMES are key transcription factors in the development of mature Natural Killer (NK) cells in mice. However, the role of these transcription factors during human NK cell development is less well understood. Therefore, we overexpressed T-BET or EOMES in human umbilical cord blood-derived HPC and cultured them in vitro in an NK cell differentiation model. To evaluate the effect of early overexpression of T-BET and EOMES in HPC, the chromatin landscape was uncovered using assay for transposase-accessible chromatin (ATAC) sequencing. In this way, the regulatory effect of T-BET and EOMES overexpression on the epigenome of HPC was evaluated.
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| Overall design |
Profiling of the chromatin accessibility landscape by Fast-ATAC sequencing of HPC transduced with transcription factors T-BET and EOMES in comparison to control transduced cells.
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| Contributor(s) |
Kiekens L, Leclercq G, Van Loocke W |
| Citation(s) |
34630413 |
| |
| Submission date |
Feb 09, 2021 |
| Last update date |
Oct 14, 2021 |
| Contact name |
Wouter Van Loocke |
| E-mail(s) |
[email protected]
|
| Organization name |
Ghent University
|
| Department |
Biomolecular Medicine
|
| Street address |
C. Heymanslaan 10
|
| City |
Ghent |
| State/province |
East Flanders |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE166439 |
ATAC-seq and RNA-seq of hematopoietic progenitor cells (HPC) and Natural Killer (NK) cells transduced with transcription factors T-BET or EOMES |
|
| Relations |
| BioProject |
PRJNA700965 |
| SRA |
SRP305494 |
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