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| Status |
Public on Aug 03, 2022 |
| Title |
Super-enhancer landscape profiling in primary colorectal carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Purpose: Examine H3K27ac enhancer and super-enhancer landscape differences between primary colorectal carcinoma and adjacent normal mucosa towards the identification of novel downstream targets.
Methods: H3K27ac ChIP-seq and RNA-seq were performed on fresh primary colorectal carcinoma samples and normal colonic mucosa, from human patient samples.
Results: We identified 2026 total super-enhancers in our cohort, between primary colorectal and normal mucosa. We quantified differences in H3K27ac signal within this space, between tumor and normal, and identified putative downstream target genes through integration with sample matched RNA-seq using a positive linear correlation model to identifty putative target genes.
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| Overall design |
Examination of H3K27ac landscape differences, particularly at super-enhancers, between primary colorectal carcinoma and patient-matched adjacent normal mucosa.
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| Contributor(s) |
Zhou RW, Parsons RE |
| Citation(s) |
36253360 |
| |
| Submission date |
Feb 05, 2021 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Royce Zhou |
| E-mail(s) |
[email protected]
|
| Organization name |
Icahn School of Medicine at Mount Sinai
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| Street address |
1470 Madison Avenue
|
| City |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
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| Samples (100)
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| Relations |
| BioProject |
PRJNA699855 |
| SRA |
SRP304890 |
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