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| Status |
Public on Dec 05, 2021 |
| Title |
Quantitative Analysis of erythroblast cells Transcriptomes treated with cholesterol or not |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In our work, we explored the dynamics of cholesterol homeostasis during terminal erythropoiesis. Interestingly, we found differential requirements of cholesterol during terminal erythropoiesis, with that cholesterol is essential for the early stage erythroblasts proliferation, while the downregulation of cholesterol biosynthesis is required for late stage cell cycle exit and final enucleation. To reveal the role of cholesterol in the the early stage erythroblasts proliferation and the detail mechanisms of erythroblasts late stage cell cycle exit, we performed RNA sequencing to analyze the gene expression profiles change between untreated and cholesterol treated erythroblasts.
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| Overall design |
TER119-negative fetal liver erythroblasts were purified and cultured in Epo containing medium for 30 h supplemented with or without cholesterol (40 μM), and total RNA was extracted using the TRIzol reagent. RNA sequencing was conducted using the BGIseq500 platform (Shenzhen, China) in triplicate.
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| Contributor(s) |
Lu Z, Zhao B |
| Citation(s) |
34739188 |
| |
| Submission date |
Feb 04, 2021 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Zhiyuan Lu |
| E-mail(s) |
[email protected]
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| Phone |
+8613220588767
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| Organization name |
Shandong First Medical University & Shandong Academy of Medical Sciences
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| Department |
School of Pharmaceutical Sciences & Institute of Materia Medica
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| Street address |
6699 Qingdao road
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| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250117 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA699475 |
| SRA |
SRP304710 |
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