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| Status |
Public on Apr 13, 2021 |
| Title |
Transneuronal Dpr12/DIP-δ interactions facilitate compartmentalized dopaminergic innervation of Drosophila mushroom body axons |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The mechanisms controlling wiring of neuronal networks are not completely understood. The stereotypic architecture of the Drosophila mushroom-body (MB) offers a unique system to study circuit assembly. The adult medial MB γ-lobe is comprised of a long bundle of axons that wires with specific modulatory and output neurons in a tiled manner defining five distinct zones. We found that the immunoglobulin superfamily protein Dpr12 is cell-autonomously required in γ-neurons for their developmental regrowth into the distal γ4/5 zones, where both Dpr12 and its interacting protein, DIP-δ, are enriched. DIP-δ functions in a subset of dopaminergic neurons that wire with γ-neurons within the γ4/5 zone. During metamorphosis, these dopaminergic projections arrive to the γ4/5 zone prior to γ-axons, suggesting that γ-axons extend through a prepatterned region. Thus, Dpr12/DIP-δ transneuronal interaction is required for γ4/5 zone formation. Our study sheds light onto molecular and cellular mechanisms underlying circuit formation within subcellular resolution.
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| Overall design |
Transcriptional profiling of drosophila γ neurons perturbed by Hr51 RNAi
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| Contributor(s) |
Bornstein B, Meltzer H, Adler R, Alyagor I, Berkun V, Cummings G, Reh F, Keren-Shaul H, David E, Riemensperger T, Schuldiner O |
| Citation(s) |
33847376 |
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| Submission date |
Feb 01, 2021 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Ido Amit |
| E-mail(s) |
[email protected]
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| Phone |
972-8-9343338
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| Organization name |
Weizmann Institute of Science
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| Department |
Immunology
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| Street address |
234 Herzl st.
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| City |
Rehovot |
| ZIP/Postal code |
760001 |
| Country |
Israel |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA698553 |
| SRA |
SRP304114 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE165896_Exp_HR51_DM6.txt.gz |
624.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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