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| Status |
Public on Nov 14, 2022 |
| Title |
Single cell atlas of epithelial and stromal cell heterogeneity by lobe and strain in the mouse prostate |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Evaluating the complex interplay of cell types in the tissue microenvironment is critical to understanding the origin and progression of diseases in the prostate and potential opportunities for intervention. Mouse models are an essential tool to investigate the molecular and cell-type-specific contributions of prostate disease at an organismal level. While there are well-documented differences in the extent, timing, and nature of disease development in various genetically engineered and exposure-based mouse models in different mouse strains and prostate lobes within each mouse strain, the underlying molecular phenotypic differences in cell types across mouse strains and prostate lobes are incompletely understood.
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| Overall design |
In this study, we used single-cell RNA-sequencing (scRNA-seq) methods to assess the single-cell transcriptomes of 6-month-old mouse prostates from two commonly used mouse strains, FVB/NJ (N = 2) and C57BL/6J (N = 3). For each mouse, the lobes of the prostate were dissected (anterior, dorsal, lateral, and ventral), and individual scRNA-seq libraries were generated.
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| Contributor(s) |
Graham MK, Chikarmane R, Wang R, Yegnasubramanian S |
| Citation(s) |
36373171 |
| |
| Submission date |
Jan 28, 2021 |
| Last update date |
Nov 18, 2022 |
| Contact name |
Mindy Kim Graham |
| E-mail(s) |
[email protected]
|
| Phone |
4104046391
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| Organization name |
Northwestern University Feinberg School of Medicine
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| Department |
Urology
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| Street address |
303 E. Superior Street Chicago
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA695577 |
| SRA |
SRP303740 |
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