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GEO help: Mouse over screen elements for information. |
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Status |
Public on Aug 05, 2021 |
Title |
Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The skin epidermis is a highly compartmentalised tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific sub compartments in the skin but none of them is IFE-specific. Here we identify Troy as a marker of IFE and HF infundibulum basal layer cells in embryonic and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells as well as their progeny confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.
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Overall design |
We generated single-cell and bulk transcriptome data from epidermal keratinocytes collected from adult murine skin epidermis.
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Contributor(s) |
Kretzschmar K, Boonekamp KE, Bleijs M, Asra P, Koomen M, Chuva de Sousa Lopes SM, Giovannone B, Clevers H |
Citation(s) |
34358453 |
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Submission date |
Jan 22, 2021 |
Last update date |
Oct 06, 2021 |
Contact name |
Kai Kretzschmar |
E-mail(s) |
[email protected]
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Organization name |
University Hospital Würzburg
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Street address |
Josef-Schneider-Str. 2
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City |
Würzburg |
ZIP/Postal code |
97080 |
Country |
Germany |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (8)
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GSM5032568 |
RNA from tdTomato+ and tdTomato- skin epidermis cells of adult Troy-EGFP-IRES-CreERT2 x Rosa26-LSL-tdTomato mice, rep1 |
GSM5032569 |
RNA from tdTomato+ and tdTomato- skin epidermis cells of adult Troy-EGFP-IRES-CreERT2 x Rosa26-LSL-tdTomato mice, rep2 |
GSM5401057 |
Extracted RNA from EGFP+ and EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 1 |
GSM5401058 |
Extracted RNA from EGFP+ and EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 2 |
GSM5401059 |
Extracted RNA from EGFP+ and EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 3 |
GSM5401060 |
Extracted RNA from EGFP+ and EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 4 |
GSM5404624 |
DAPI-, ITGA6bright EGFP+ and ITGA6bright EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 1 |
GSM5404625 |
DAPI-, ITGA6bright EGFP+ and ITGA6bright EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 2 |
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Relations |
BioProject |
PRJNA694196 |
SRA |
SRP302937 |
Supplementary file |
Size |
Download |
File type/resource |
GSE165379_CEL-Seq2_UMI_barcodes.csv.gz |
2.1 Kb |
(ftp)(http) |
CSV |
GSE165379_RAW.tar |
3.6 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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