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Series GSE165379 Query DataSets for GSE165379
Status Public on Aug 05, 2021
Title Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The skin epidermis is a highly compartmentalised tissue consisting of a cornifying epithelium called the interfollicular epidermis (IFE) and associated hair follicles (HFs). Several stem cell populations have been described that mark specific sub compartments in the skin but none of them is IFE-specific. Here we identify Troy as a marker of IFE and HF infundibulum basal layer cells in embryonic and adult human and mouse epidermis. Genetic lineage-tracing experiments demonstrate that Troy-expressing basal cells contribute to long-term renewal of all layers of the cornifying epithelium. Single-cell transcriptomics and organoid assays of Troy-expressing cells as well as their progeny confirmed stem cell identity as well as the ability to generate differentiating daughter cells. In conclusion, we define Troy as a marker of epidermal basal cells that govern interfollicular epidermal renewal and cornification.
 
Overall design We generated single-cell and bulk transcriptome data from epidermal keratinocytes collected from adult murine skin epidermis.
 
Contributor(s) Kretzschmar K, Boonekamp KE, Bleijs M, Asra P, Koomen M, Chuva de Sousa Lopes SM, Giovannone B, Clevers H
Citation(s) 34358453
Submission date Jan 22, 2021
Last update date Oct 06, 2021
Contact name Kai Kretzschmar
E-mail(s) [email protected]
Organization name University Hospital Würzburg
Street address Josef-Schneider-Str. 2
City Würzburg
ZIP/Postal code 97080
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM5032568 RNA from tdTomato+ and tdTomato- skin epidermis cells of adult Troy-EGFP-IRES-CreERT2 x Rosa26-LSL-tdTomato mice, rep1
GSM5032569 RNA from tdTomato+ and tdTomato- skin epidermis cells of adult Troy-EGFP-IRES-CreERT2 x Rosa26-LSL-tdTomato mice, rep2
GSM5401057 Extracted RNA from EGFP+ and EGFP- cells of skin epidermis of adult Troy-EGFP-IRES-CreERT2 mice, replicate 1
Relations
BioProject PRJNA694196
SRA SRP302937

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165379_CEL-Seq2_UMI_barcodes.csv.gz 2.1 Kb (ftp)(http) CSV
GSE165379_RAW.tar 3.6 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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