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Series GSE164878 Query DataSets for GSE164878
Status Public on Sep 24, 2021
Title Rat transcriptomic profiling of liver sinusoidal endothelial cells during chronic liver disease reveals stage-specific secretory signature
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary RNAseq was applied to define the transcriptome of primary LSECs from rats with acute liver injury, mild liver fibrosis and advanced chronic liver disease (CLD) (due to CCl4 or thioacetamide administration). Results showed different transcriptomic profile during progression of CLD, and a large number of significant de-regulated genes (1412) were shared in cirrhotic CCl4- and TAA-LSECs (fold-change>1.5 and p-vale<0.05)compared with their respective healthy-LSECs.
 
Overall design RNA-seq transcriptomic profiles were derived from liver sinusoidal endothelial cells (LSECs) isolated during chronic liver disease (CLD). Wistar Han rats underwent inhalation exposure to carbon tetrachloride (CCl4) for 2 weeks (n=5), 6 weeks (n=5) or 12-14 weeks (n=6). Healthy LSECs were isolated of Wistar Han animals (n=6). Additionally, RNA-seq was performed from LSECs isolated of healthy Sprague-Dawley rats (n=5) and of TAA-cirrhotic rats (n=5).
 
Contributor(s) Fernandez-Iglesias A, Gracia-Sancho J
Citation(s) 34072510
Submission date Jan 14, 2021
Last update date Sep 25, 2021
Contact name Juanjo Lozano
E-mail(s) [email protected]
Organization name CIBEREHD
Department Plataforma de Bioinformatica
Street address C/ Rosselló 153
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (32)
GSM5022280 Liver sinusoidal endothelial cells : CH1
GSM5022281 Liver sinusoidal endothelial cells : CH2
GSM5022282 Liver sinusoidal endothelial cells : CH3
Relations
BioProject PRJNA692264
SRA SRP301855

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Supplementary file Size Download File type/resource
GSE164878_RAW.tar 8.4 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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