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| Status |
Public on Mar 18, 2010 |
| Title |
Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal of this experiment was to identify the early target genes of constitutively activated CPK5ac and CPK11ac in Arabidopsis mesophyll cells that are involved in early flagellin responses important for plant innate immunity.
We used the microarrays to detail the global program of gene expression underlying initial calcium responses to microbial associated molecualr patterns (MAMPs).
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| Overall design |
Arabidopsis mesophyll protoplasts were transfected with plasmid DNA to express constitutively active CPK5ac or CPK11ac for 6 hr. Transcript profiles were analyzed and compared between cells transfected with control DNA and CPK5ac or CPK11ac DNA to identify early CPK5ac and CPK11ac target genes.
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| Web link |
http://www.nature.com.ezp-prod1.hul.harvard.edu/nature/journal/v464/n7287/suppinfo/nature08794.html
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| Contributor(s) |
Sheen J, Willmann M, Boudsocq M, Lee H, McCormack M |
| Citation(s) |
20164835 |
| |
| Submission date |
Jun 06, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Jen Sheen |
| E-mail(s) |
[email protected]
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Sheen
|
| Street address |
185 Cambridge st. CPZN7620
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114-2790 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM413936 |
protoplasts at T0, biological rep1 [CPK] |
| GSM413937 |
protoplasts at T1, biological rep1 [CPK] |
| GSM413938 |
protoplasts at T2, biological rep1 [CPK] |
| GSM413939 |
protoplasts at T0, biological rep2 [CPK] |
| GSM413940 |
protoplasts at T1, biological rep2 [CPK] |
| GSM413941 |
protoplasts at T2, biological rep2 [CPK] |
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| This SubSeries is part of SuperSeries: |
| GSE16557 |
Gene expression changes underlying initial cellular and molecular responses to MAMPs in Arabidopsis mesophyll cells |
|
| Relations |
| BioProject |
PRJNA122903 |
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