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| Status |
Public on May 18, 2021 |
| Title |
RNAseq in JARID2 siRNA-treated in vitro differentiated human primary adipocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNAseq was performed in siJARID2 and control siRNA-treated human adipose tissue derived stem cells (hASC). The tretment of siRNA was performed by electroporation one day before induction of differentiation in vitro. The cells were lyzed and RNA was purified on day 6 (mid differentiation) and day 13 (full differentiation) from differentiation start.
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| Overall design |
RNAseq was performed in siJARID2 and control siRNA-treated human adipose tissue derived stem cells (hASC). The tretment of siRNA was performed by electroporation one day before induction of differentiation in vitro. The cells were lyzed and RNA was purified on day 6 (mid differentiation) and day 13 (full differentiation) from differentiation start. Three independen experiments were analyzed for both day 6 (transfections HG23, HG23_2, HG24) and day 13 (transfections TRANSF2,3,4). Samples of negative control (non-targeting siRNA) and siJARID2 from the same experiment were anayzed as paired samples.
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| Contributor(s) |
Subramanian N, Laurencikiene J, Björk C |
| Citation(s) |
33963396 |
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| Submission date |
Jan 07, 2021 |
| Last update date |
May 23, 2021 |
| Contact name |
Jurga Laurencikiene |
| Organization name |
Karolinska Institutet
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| Street address |
Hälsovägen 7
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| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA690568 |
| SRA |
SRP300809 |
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