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| Status |
Public on Dec 01, 2023 |
| Title |
3' RNA-seq of brains from control and circMbl KD flies |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine functions of circMbl in brain in vivo we generated genetically encoded shRNA directed against individual circRNA-specific back-spliced junctions. We analyzed gene expression by 3' RNA-seq from control (actin-Gal4) and flies expressing the shRNA under the control of a constitutive driver (actin-Gal4; circRNA KD).
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| Overall design |
Brains from eight miss match (8MM) sh control flies and circMbl KD flies were disected. 5 control samples and 2 KD fly samples were sequenced and procesed
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| Contributor(s) |
Kadener S |
| Citation missing |
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| |
| Submission date |
Dec 23, 2020 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
[email protected]
|
| Organization name |
Brandeis University
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| Department |
Biology department
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA687547 |
| SRA |
SRP299169 |
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