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Series GSE161498 Query DataSets for GSE161498
Status Public on Dec 15, 2020
Title Transcriptional effect of early-life adversity in adult hippocampus
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Early-life adversity (ELA) is associated with lifelong memory deficits, yet the responsible mechanisms remain unclear. We imposed ELA by rearing rat pups in simulated poverty, assessed hippocampal memory, and probed changes in gene expression, their transcriptional regulation and the consequent changes in hippocampal neuronal structure. ELA rats had poor hippocampal memory and stunted hippocampal pyramidal neurons, associated with ~140 differentially expressed genes. Upstream regulators of the altered genes included glucocorticoid receptor and, unexpectedly, the transcription factor neuron-restrictive silencer factor (NRSF/REST). NRSF contributed critically to the memory deficits because blocking its function transiently following ELA rescued spatial memory and restored the dendritic arborization of hippocampal pyramidal neurons in ELA rats. Blocking NRSF function in vitro augmented dendritic complexity of developing hippocampal neurons, suggesting that NRSF represses genes involved in neuronal maturation. These findings establish important, surprising contributions of NRSF to ELA-induced transcriptional programming that disrupts hippocampal maturation and memory function.
 
Overall design Gene expression was profiled in dorsal hippocampi from 8-week-old male rats that were subjected to either control rearing conditions (n=4) or ELA (n=5), and did not undergo behavioral testing.
 
Contributor(s) Schulmann A, Bolton JL, Regev L, Jiang S, Chen Y, Molet J, Garcia-Curran MM, Singh-Taylor A, Kamei N, Shao M, Noam Y, Mortazavi A, Baram TZ
Citation(s) 33326786
Submission date Nov 15, 2020
Last update date Jan 26, 2021
Contact name Anton Schulmann
E-mail(s) [email protected]
Organization name NIH/NIMH
Lab McMahon
Street address 35 Convent Dr
City Bethesda
State/province Maryland (MD)
ZIP/Postal code 20814
Country USA
 
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (9)
GSM4908785 CTL_rep1
GSM4908786 CTL_rep2
GSM4908787 CTL_rep3
Relations
BioProject PRJNA678522
SRA SRP292611

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161498_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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