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Status |
Public on Jun 12, 2009 |
Title |
Transcriptome comparison of Col-0, se-1, ago1-27 and se-1 35S::MIR156 in Arabidopsis thaliana. |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
Microarray analysis of wild type plants and plants with reduced (ago1-27 and se-1) or increased miR156 levels (se-1 p35S:MIR156). Shoot apices were dissected from 20-day-old, short-day grown plants.
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Overall design |
Plants are grown in short day for 20 days. Total RNAs from shoot apex are extracted by RNeasy Plant Mini kit (Qiagen). The probes are labeled with MessageAmp-Biotin Enhanced, single round aRNA amplification kit from Ambion and hybridized to Affy ATH1 array. Each genotype has two replicates.
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Contributor(s) |
Weigel D, Wang J |
Citation(s) |
19703399 |
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Submission date |
May 12, 2009 |
Last update date |
Aug 28, 2018 |
Contact name |
Jiawei Wang |
E-mail(s) |
[email protected]
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Organization name |
Max Planck Institute for Developmental Biology
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Street address |
Spemannstr 37-39
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City |
Tuebingen |
ZIP/Postal code |
D-72076 |
Country |
Germany |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (8)
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GSM402170 |
Arabidopsis thaliana_apex_SD_20-day_Col-0_replicate 1 |
GSM402171 |
Arabidopsis thaliana_apex_SD_20-day_Col-0_replicate 2 |
GSM402172 |
Arabidopsis thaliana_apex_SD_20-day_ago1-27_replicate 1 |
GSM402173 |
Arabidopsis thaliana_apex_SD_20-day_ago1-27_replicate 2 |
GSM402174 |
Arabidopsis thaliana_apex_SD_20-day_se-1_replicate 1 |
GSM402175 |
Arabidopsis thaliana_apex_SD_20-day_se-1_replicate 2 |
GSM402176 |
Arabidopsis thaliana_apex_SD_20-day_se-1 p35S::MIR156_replicate 1 |
GSM402177 |
Arabidopsis thaliana_apex_SD_20-day_se-1 p35S::MIR156_replicate 2 |
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Relations |
BioProject |
PRJNA115479 |