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| Status |
Public on Dec 18, 2021 |
| Title |
The Asymmetric Distribution of RNAPII and Nucleosomes on Replicated Daughter Genomes is caused by Differences in Replication Timing between the Lagging and the Leading Strand [ChIP] |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Chromatin features are thought to have a role in the epigenetic transmission of transcription states from one cell generation to the next. It is unclear how chromatin structure survives disruptions caused by genomic replication or if chromatin features are instructive of the transcription state of the underlying gene. We developed a method to monitor budding yeast replication, transcription and chromatin maturation dynamics on each daughter genome in parallel, with which we identified clusters of secondary origins surrounding known origins. We find a difference in the timing of lagging and leading strand replication on the order of minutes at most yeast genes. We propose a model in which the majority of old histones and RNAPII bind to the gene copy that replicated first, while newly synthesized nucleosomes are assembled on the copy that replicated second. RNAPII enrichment then shifts to the sister copy that replicated second. The order of replication is largely determined by genic orientation: if transcription and replication are co-directional the leading strand replicates first; if they are counter-directional the lagging strand replicates first. A mutation in the Mcm2 subunit of the replicative helicase Mcm2-7 which impairs Mcm2 interactions with histone H3 slows down replication forks but does not qualitatively change the asymmetry in nucleosome distribution observed in the WT. Active transcription states are inherited simultaneously and independently of their underlying chromatin states through the recycling of the transcription machinery and old histones, respectively. Transcription thus actively contributes to the reestablishment of the active chromatin state.
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| Overall design |
two Rpb3-HA ChIP samples on sonicated chromatin from S-phase cells 25 and 32 min after release from alpha factor G1 arrest with a dye flip technical replicate foreach sample
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| Contributor(s) |
Ziane R, Camasses A, Radman-Livaja M |
| Citation(s) |
35042724 |
| |
| Submission date |
Oct 30, 2020 |
| Last update date |
Mar 20, 2022 |
| Contact name |
Marta Radman-Livaja |
| E-mail(s) |
[email protected]
|
| Phone |
+33434359667
|
| Organization name |
CNRS
|
| Department |
IGMM
|
| Street address |
1919 route de Mende
|
| City |
Montpellier |
| ZIP/Postal code |
34293 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL10930 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) [Probe Name version] |
|
| Samples (4)
|
| GSM4874318 |
Rpb3-HA (subunit of RNAPol2) ChIP 25min after release from G1 |
| GSM4874319 |
Rpb3-HA (subunit of RNAPol2) ChIP 32min after release from G1 |
| GSM4874320 |
Rpb3-HA (subunit of RNAPol2) ChIP 25min after release from G1 dye flip |
| GSM4874321 |
Rpb3-HA (subunit of RNAPol2) ChIP 32min after release from G1 dye flip |
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| This SubSeries is part of SuperSeries: |
| GSE160509 |
The Asymmetric Distribution of RNAPII and Nucleosomes on Replicated Daughter Genomes is caused by Differences in Replication Timing between the Lagging and the Leading Strand |
|
| Relations |
| BioProject |
PRJNA673419 |
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