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Series GSE160187 Query DataSets for GSE160187
Status Public on Oct 28, 2020
Title Do Copper enhance antibiotic resistance (AR) in resistant bacteria and/or induce AR in sensitive bacteria? [RNA-seq]
Organism Pseudomonas aeruginosa
Experiment type Expression profiling by high throughput sequencing
Summary The aim of this experiment was to determine if the development of resistance to antibiotics can be driven by the concentration and speciation of Cu. Experimental setup was designed to investigate two hypotheses for which two strains of Gram- bacteria have been selected: - Do TE enhance AR in resistant bacteria? Resistant strain: Bioluminescent Pseudomonas aeruginosa PAO1 (Xen41, Tetracycline resistant) - Do TE induce AR in sensitive bacteria? Sensitive strain: Pseudomonas aeruginosa PAO1 (Wild Type)
 
Overall design A 72h exposure hydroponic experiment has been carried out. The growth media was selected to provide optimum free Cu2+ concentration in solution by limiting the complexation of Cu2+ by the constituents in the medium. This media was spiked with three Cu concentrations: Cu=0 (control) Cu=MIC/10 and Cu=MIC (MIC= minimal inhibitory concentration). Samples (bacteria + solution) have been collected after 0, 24, 48 and 72 hours.
 
Contributor(s) Doelsch E, Ogunniyi D, Breen J
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Submission date Oct 27, 2020
Last update date Oct 28, 2020
Contact name James Breen
E-mail(s) [email protected]
Organization name Australian National University
Department John Curtin School of Medical Research
Lab Black Ochre Data Labs (Adelaide)
Street address 108 North Terrace (Ground Floor)
City Adelaide
State/province SA
ZIP/Postal code 5000
Country Australia
 
Platforms (1)
GPL21297 Illumina NextSeq 500 (Pseudomonas aeruginosa)
Samples (60)
GSM4862051 PAO1_control_Exp3_t=0 (RNA-seq)
GSM4862052 PAO1_control_Exp3_t=24 (RNA-seq)
GSM4862053 PAO1_control_Exp3_t=48 (RNA-seq)
This SubSeries is part of SuperSeries:
GSE160190 Do Copper enhance antibiotic resistance (AR) in resistant bacteria and/or induce AR in sensitive bacteria?
Relations
BioProject PRJNA672286
SRA SRP288730

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE160187_countTable.tsv.gz 603.3 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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