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Series GSE15876 Query DataSets for GSE15876
Status Public on Jul 19, 2010
Title The SHR/SCR pathway directly activates genes involved in asymmetric cell division in the Arabidopsis root
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Asymmetric division of cortex/endodermal initials (CEI) in the Arabidopsis root generates two layers of ground tissue and is controlled by a finely orchestrated interplay between the transcription factors, SHORT ROOT (SHR) and SCARECROW (SCR). To understand the dynamics of the SHR/SCR regulatory network we performed microarray time course experiments using inducible versions of SHR and SCR and examined their transcriptional effects specifically in the ground tissue.

Keywords: SHR and SCR time course and CEI cell-type analyses
 
Overall design We performed a time course analysis (TC data set) of the ground tissue (sorted J0571 enancher trap) after transfer either SHR or SCR inducible plants to Dex containing plates 0, 1, 3, 6 and 12 hours. We sorted for CEI cells using the pCYCD6::GFP marker line. Seedlings were grown for 5 days. SHR and SCR inducible plants were transfer to 1microM Dex MS plates. J0571 and CYCD6 sorted fluorecent cells were harvested for RNA extraction.
 
Contributor(s) Sozzani R, Benfey PN
Citation(s) 20596025
Submission date Apr 28, 2009
Last update date Aug 15, 2018
Contact name Rosangela Sozzani
E-mail(s) [email protected]
Phone 919 6138202
Organization name Duke University
Department Biology
Lab Benfey
Street address 124 Science Dr
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (23)
GSM398673 Arabidopsis ATH1_SHR inducible line_time course_first biological replicate Time 0H after Dex treatment
GSM398674 Arabidopsis ATH1_SHR inducible line_time course_first biological replicate Time 1H after Dex treatment
GSM398675 Arabidopsis ATH1_SHR inducible line_time course_first biological replicate Time 3H after Dex treatment
Relations
Affiliated with GSE69995
BioProject PRJNA117031

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15876_RAW.tar 70.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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