|
| Status |
Public on Dec 01, 2020 |
| Title |
Non-templated oligo-adenylation of small RNAs in the parasite Entamoeba |
| Organisms |
Entamoeba histolytica; Entamoeba invadens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Other
|
| Summary |
we found a new population of 31nt sRNAs that results from the addition of a non-templated 3-4 adenosine nucleotides at the 3´-end of the 27nt sRNA populations. We sequenced various sRNA libraries including both total RNAs and IP-ed RNAs of EhAgo proteins.
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| |
|
| Overall design |
sRNA library from size-selected (30-45nt) total RNA of E. histolytica trophozoites; from size-selected (30-45nt) total RNAs of development of E. invadens; from IP-ed RNAs of EhAgo proteins and mutant.
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| |
|
| Contributor(s) |
Zhang H, Ehrenkaufer G, Hall N, Singh U |
| Citation(s) |
33297948 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI121084 |
Small RNA regulation of gene expression in Entamoeba |
STANFORD UNIVERSITY |
UPINDER SINGH |
|
| |
| Submission date |
Sep 09, 2020 |
| Last update date |
Dec 15, 2020 |
| Contact name |
Gretchen Marie Ehrenkaufer |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford Uninversity
|
| Department |
Microbiology and Immunology
|
| Lab |
Singh
|
| Street address |
Grant S-143, 300 Pasteur Dr.
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19933 |
Illumina MiSeq (Entamoeba histolytica) |
| GPL19934 |
Illumina MiSeq (Entamoeba invadens) |
|
| Samples (13)
|
| GSM4774619 |
E. invadens, excyst 8hr [IP-1_excyst8_35] |
| GSM4774620 |
anti-Myc IP of Myc-EhAgo2-1, total lysate [IPAgo1_RppH] |
| GSM4774621 |
anti-Myc IP of Myc-EhAgo2-2, total lysate [IPAgo2_RppH] |
| GSM4774622 |
anti-Myc IP of Myc-EhAgo2-3, total lysate [IPAgo3_RppH] |
| GSM4774623 |
anti-Myc IP of Myc-EhAgo2-2△NLS-DR, total lysate [IPAgo2_mut_RppH] |
| GSM4774624 |
anti-Myc IP of Myc-EhAgo2-2, cytosolic lysate [IPAgo2_cyto_RppH] |
| GSM4774625 |
anti-Myc IP of Myc-EhAgo2-2, nuclear lysate [IPAgo2_nuc_RppH] |
| GSM4774626 |
anti-Myc IP of Myc-EhAgo2-1, total lysate [IPAgo1_TAP] |
| GSM4774627 |
anti-Myc IP of Myc-EhAgo2-2, total lysate [IPAgo2_TAP] |
| GSM4774628 |
anti-Myc IP of Myc-EhAgo2-3, total lysate [IPAgo3_TAP] |
|
| Relations |
| BioProject |
PRJNA662625 |
| SRA |
SRP281913 |
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