 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 06, 2021 |
| Title |
mRNA-seq in VDR knockout and control THP-1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The vitamin D receptor (VDR) has been knocked out in monocytic THP-1 cells after stimulation of ko and control cells with the the natural VDR ligand 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), quadruplicate mRNA-seq has been performed
|
| |
|
| Overall design |
The knockout has been performed by lentiviral CRISPR/Cas9 gene editing using a guideRNA (gRNA) directed against the VDR gene. The control cells have been created using a non-targeting gRNA (NTC). Prior to MRNA-seq, the cells were treated for 24 h with 100 nM 1,25(OH)2D3 or the solvent control (EtOH).
|
| |
|
| Contributor(s) |
Warwick T, Seuter S, Schulz MH, Brandes RP |
| Citation(s) |
33753848 |
| |
| Submission date |
Sep 04, 2020 |
| Last update date |
Dec 06, 2021 |
| Contact name |
Timothy Warwick |
| E-mail(s) |
[email protected]
|
| Organization name |
Goethe Universität Frankfurt am Main
|
| Department |
Institute for Cardiovascular Physiology
|
| Street address |
Theodor-Stern-Kai 7
|
| City |
Frankfurt am Main |
| State/province |
Hesse |
| ZIP/Postal code |
60590 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (32)
|
| GSM4769124 |
control THP-1, 4 h EtOH, replicate 1 |
| GSM4769125 |
control THP-1, 4 h 1,25(OH)2D3, replicate 1 |
| GSM4769126 |
control THP-1, 24 h EtOH, replicate 1 |
| GSM4769127 |
control THP-1, 24 h 1,25(OH)2D3, replicate 1 |
| GSM4769128 |
VDR ko THP-1, 4 h EtOH, replicate 1 |
| GSM4769129 |
VDR ko THP-1, 4 h 1,25(OH)2D3, replicate 1 |
| GSM4769130 |
VDR ko THP-1, 24 h EtOH, replicate 1 |
| GSM4769131 |
VDR ko THP-1, 24 h 1,25(OH)2D3, replicate 1 |
| GSM4769132 |
control THP-1, 4 h EtOH, replicate 2 |
| GSM4769133 |
control THP-1, 4 h 1,25(OH)2D3, replicate 2 |
| GSM4769134 |
control THP-1, 24 h EtOH, replicate 2 |
| GSM4769135 |
control THP-1, 24 h 1,25(OH)2D3, replicate 2 |
| GSM4769136 |
VDR ko THP-1, 4 h EtOH, replicate 2 |
| GSM4769137 |
VDR ko THP-1, 4 h 1,25(OH)2D3, replicate 2 |
| GSM4769138 |
VDR ko THP-1, 24 h EtOH, replicate 2 |
| GSM4769139 |
VDR ko THP-1, 24 h 1,25(OH)2D3, replicate 2 |
| GSM4769140 |
control THP-1, 4 h EtOH, replicate 3 |
| GSM4769141 |
control THP-1, 4 h 1,25(OH)2D3, replicate 3 |
| GSM4769142 |
control THP-1, 24 h EtOH, replicate 3 |
| GSM4769143 |
control THP-1, 24 h 1,25(OH)2D3, replicate 3 |
| GSM4769144 |
VDR ko THP-1, 4 h EtOH, replicate 3 |
| GSM4769145 |
VDR ko THP-1, 4 h 1,25(OH)2D3, replicate 3 |
| GSM4769146 |
VDR ko THP-1, 24 h EtOH, replicate 3 |
| GSM4769147 |
VDR ko THP-1, 24 h 1,25(OH)2D3, replicate 3 |
| GSM4769148 |
control THP-1, 4 h EtOH, replicate 4 |
| GSM4769149 |
control THP-1, 4 h 1,25(OH)2D3, replicate 4 |
| GSM4769150 |
control THP-1, 24 h EtOH, replicate 4 |
| GSM4769151 |
control THP-1, 24 h 1,25(OH)2D3, replicate 4 |
| GSM4769152 |
VDR ko THP-1, 4 h EtOH, replicate 4 |
| GSM4769153 |
VDR ko THP-1, 4 h 1,25(OH)2D3, replicate 4 |
| GSM4769154 |
VDR ko THP-1, 24 h EtOH, replicate 4 |
| GSM4769155 |
VDR ko THP-1, 24 h 1,25(OH)2D3, replicate 4 |
|
| Relations |
| BioProject |
PRJNA661550 |
| SRA |
SRP280139 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE157514_VDR.KO.RNAseq.ResultsTables.4N.xlsx |
64.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...