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| Status |
Public on Aug 04, 2020 |
| Title |
Single-cell RNA sequencing of Cd45+ adventitial cells from mice at steady state or treated with AngiotensinII |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we show, that arterial macrophages derive predominantly from YS EMPs, which give rise to a transcriptionally distinct cluster of macrophages with mainly homeostatic and anti-inflammatory properties in steady state as well as in response to AngII inflammation. In adult mice, these EMP-derived macrophages persist for long periods of time, but decreas in absolute numbers in senescence without compensation by BM-derived progenitors. EMP-derived macrophages expressed high levels of homeostatic genes such as Lyve-1, which links them to physiological functions in arteries.
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| Overall design |
Examination of adventitial immune cells in RankCreRosa26eYFP in two conditions (steady state and AnII-induced inflammation)
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| Contributor(s) |
Weinberger T, Esfandyari D, Engelhardt S, Schulz C |
| Citation(s) |
32917889 |
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| Submission date |
Aug 03, 2020 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Dena Esfandyari |
| E-mail(s) |
[email protected], [email protected]
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| Phone |
08941403286
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| Organization name |
Technical University of Munich
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| Department |
Institute of Pharmacology and Toxicology
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| Street address |
Haus 607, Biedersteiner Str. 29, IPT
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| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
80802 |
| Country |
Germany |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA650317 |
| SRA |
SRP275591 |
