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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 03, 2022 |
| Title |
Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Biliary diseases can cause inflammation, fibrosis, bile duct destruction and eventually liver failure. There are no curative treatments for biliary disease except for liver transplantation. New therapies are urgently required.
We have purified human Biliary Epithelial Cells (hBEC) from discarded human livers. hBECs were tested as a cell therapy in a mouse model of biliary disease where the conditional deletion of Mdm2 in cholangiocytes causes senescence, biliary strictures and fibrosis.
hBEC are expandable, phenotypically stable and help restore biliary structure and function, highlighting their regenerative capacity and a potential alternative to liver transplantation for biliary disease.
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| Overall design |
Differential expression of CD133+ and CD133- human biliary epithelial cells from healthy (n=2) and steatotic (n=3) livers; Gene expression at different cell passages of CD133+ human biliary epithelial cells grown in vitro in two dimension-GMP compatible conditions (2D; n=4) and three dimensions (3D, organoids; n=5)
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| Contributor(s) |
Hallett JM, Ferreira-Gonzalez S, Man TY, Kilpatrick AM, Esser H, Thirlwell K, MacMillan M, Rodrigo-Torres D, Dwyer BJ, Gadd VL, Ashmore-Harris C, Lu W, Thomson J, Jansen MA, O'Duibhir E, Starkey Lewis PJ, Campana L, Aird R, Bate T, Fraser AR, Campbell JD, Oniscu GC, Hay D, Callanan A, Forbes SJ |
| Citation(s) |
35245467 |
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| Submission date |
Jul 31, 2020 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Stuart Forbes |
| Organization name |
The University of Edinburgh
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| Department |
Centre for Regenerative Medicine
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| Street address |
5 Little France Drive
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| City |
Edinburgh |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
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| Platforms (2) |
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| Samples (19)
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| GSM4705318 |
Biliary epithelial cells - HL5 CD133- |
| GSM4705319 |
Biliary epithelial cells - HL6 CD133+ |
| GSM4705320 |
Biliary epithelial cells - HL6 CD133- |
| GSM4705321 |
Biliary epithelial cells - HL9 CD133+ |
| GSM4705322 |
Biliary epithelial cells - HL9 CD133- |
| GSM4705323 |
Biliary epithelial cells - HL11 CD133+ |
| GSM4705324 |
Biliary epithelial cells - HL11 CD133- |
| GSM5527664 |
Biliary epithelial cells - HL29 CD133+ P0 cultured in 2D |
| GSM5527665 |
Biliary epithelial cells - HL29 CD133+ P1 cultured in 2D |
| GSM5527666 |
Biliary epithelial cells - HL29 CD133+ P3 cultured in 2D |
| GSM5527667 |
Biliary epithelial cells - HL29 CD133+ P5 cultured in 2D |
| GSM5527668 |
Biliary epithelial cells - HL29 CD133+ P0 cultured in 3D |
| GSM5527669 |
Biliary epithelial cells - HL29 CD133+ P2 cultured in 3D |
| GSM5527670 |
Biliary epithelial cells - HL29 CD133+ P5 cultured in 3D |
| GSM5527671 |
Biliary epithelial cells - HL29 CD133+ P8 cultured in 3D |
| GSM5527672 |
Biliary epithelial cells - HL29 CD133+ P14 cultured in 3D |
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| Relations |
| BioProject |
PRJNA649950 |
| SRA |
SRP274582 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155498_2d3d_gene_counts_matrix.txt.gz |
469.6 Kb |
(ftp)(http) |
TXT |
| GSE155498_hBEC_gene_counts_matrix.txt.gz |
428.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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