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| Status |
Public on Nov 30, 2020 |
| Title |
Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
U937 cells, human lyphoma cell line, differentiate to the macrophage-like cells by the phorbol 12-myristate 13-acetate (PMA). We investigated the regulation mechanisms of gene expression during the U937 differentiation by RNA-seq.
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| Overall design |
We cultured the U937 cells with or without 10 nM PMA. Total RNA was extracted from cells and polyA(+) RNA was concentrated by oligo-dT conjugated dynabeads. RNA-seq were performed by Ion Proton Platform.
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| Contributor(s) |
Ohse K |
| Citation missing |
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| |
| Submission date |
Jul 30, 2020 |
| Last update date |
Nov 30, 2020 |
| Contact name |
Kensuke Ohse |
| E-mail(s) |
[email protected]
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| Organization name |
Tokyo Metropolitan Institute of Gerontology
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| Street address |
Sakaecho
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| City |
Itabashi-ku |
| ZIP/Postal code |
173-0015 |
| Country |
Japan |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA649795 |
| SRA |
SRP274388 |
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