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| Status |
Public on Oct 13, 2021 |
| Title |
Tumor-reactive tissue resident memory T cells, regulatory T cells and prognosis in early luminal-like breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Luminal-like breast cancer (BC) constitutes the majority of BC subtypes, but, differently from highly aggressive triple negative breast cancer (TNBC), is poorly infiltrated by the immune system. The quality of the immune infiltrate in luminal-like BCs has been poorly studied, thus a biological basis for the further investigation of immunotherapeutic strategies is missing. By using high-dimensional single-cell technologies, we identified heterogeneous behavior within the tissue-resident memory CD8+ T (Trm) cells infiltrating luminal-like tumors. We defined that a subset of CD127- CD39hi Trm cells is preferentially present in the tumor compared to the adjacent normal breast tissue or peripheral blood, displayed enhanced effector functions compared to the CD127+ CD39lo Trm counterpart, and was specifically associated with positive prognosis. Nevertheless, the prognostic benefit was lost in the presence of highly-suppressive CCR8hi ICOShi IRF4+ effector Tregs. Our results suggest that combinatorial strategies aiming at boosting Trm function while relieving from Treg-mediated immunosuppression are needed to achieve proper tumor control in luminal-like BCs.
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| |
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| Overall design |
RNA-Seq profiling of CD127+ CD39low and CD127- CD39high CD8+ Trm cells
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| |
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| Contributor(s) |
Losurdo A, Scirgolea C, Alvisi G, Mazza EM, Lugli E |
| Citation(s) |
34552178 |
| |
| Submission date |
Jul 21, 2020 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Emilia Maria Cristina Mazza |
| E-mail(s) |
[email protected]
|
| Organization name |
Humanitas Clinical and Research Center
|
| Street address |
Via Alessandro Manzoni, 56
|
| City |
Rozzano, Milan |
| ZIP/Postal code |
20089 |
| Country |
Italy |
| |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA647774 |
| SRA |
SRP273141 |
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